peptone supplementation of culture medium has variable effects on the productivity of cho cells

نویسندگان

fatemeh davami laboratory of cellular biotechnology, ècole polytechnique fédérale de lausanne, switzerland.

lucia baldi laboratory of cellular biotechnology, ècole polytechnique fédérale de lausanne, switzerland.

yashas rajendra laboratory of cellular biotechnology, ècole polytechnique fédérale de lausanne, switzerland.

florian m. wurm laboratory of cellular biotechnology, ècole polytechnique fédérale de lausanne, switzerland.

چکیده

the optimization of cell culture conditions for growth and productivity of recombinant chinese hamster ovary (cho) cells is a critical step in biopharmaceutical manufacturing. in the present study, the effects of the timing and amount of peptone feeding of a recombinant cho cell line grown in a basal medium in serum-free suspension culture were determined for eight peptones of different origin (plant and casein). the amino acid content and the average molecular weight of the peptones chosen were available. in optimized feeding strategies with single peptones, increase 100 % volumetric productivity and 40 % in cell number were achieved. in feeding strategies with two peptones, several combinations stimulated protein productivity more than either peptone alone, depending on the peptone concentration and time of feeding. some peptones, which did not stimulate productivity when added alone proved to be effective when used in combination. the combined peptones feeding strategies were more effective with peptones of different origin. our data support the notion that the origin of peptones provides some guidance in identifying the most effective feeding strategies for recombinant cho cells.

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منابع مشابه

Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells

The optimization of cell culture conditions for growth and productivity of recombinant Chinese hamster ovary (CHO) cells is a critical step in biopharmaceutical manufacturing. In the present study, the effects of the timing and amount of peptone feeding of a recombinant CHO cell line grown in a basal medium in serum-free suspension culture were determined for eight peptones of different origin ...

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Peptone Supplementation of Culture Medium Has Variable Effects on the Productivity of CHO Cells

The optimization of cell culture conditions for growth and productivity of recombinant Chinese hamster ovary (CHO) cells is a critical step in biopharmaceutical manufacturing. In the present study, the effects of the timing and amount of peptone feeding of a recombinant CHO cell line grown in a basal medium in serum-free suspension culture were determined for eight peptones of different origin ...

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Effects of Peptone Supplementation in Different Culture Media on Growth, Metabolic Pathway and Productivity of CHO DG44 Cells a New Insight into Amino Acid Profiles

Background: The optimization of bioprocess conditions towards improved growth profile and productivity yield is considered of great importance in biopharmaceutical manufacturing. Peptones as efficient sources of nutrients have been studied for their effect on media development however, their role on metabolic pathway is not well understood. Methods: In the present study, the effect of different...

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Effects of Peptone Supplementation in Different Culture Media on Growth, Metabolic Pathway and Productivity of CHO DG44 Cells; a New Insight into Amino Acid Profiles

BACKGROUND The optimization of bioprocess conditions towards improved growth profile and productivity yield is considered of great importance in biopharmaceutical manufacturing. Peptones as efficient sources of nutrients have been studied for their effect on media development; however, their role on metabolic pathway is not well understood. METHODS In the present study, the effect of differen...

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effects of peptone supplementation in different culture media on growth, metabolic pathway and productivity of cho dg44 cells a new insight into amino acid profiles

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عنوان ژورنال:
international journal of molecular and cellular medicine

جلد ۳، شماره ۳، صفحات ۱۴۶-۱۵۶

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